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Abstract
Background: In addition to virulence factors, the ability to resist antibiotics has helped A. baumannii become the most common cause of hospital-acquired infection. Studies on carbapenem resistance mechanisms in Vietnam have also focused on the common mechanism of carbapenemase enzyme resistance. Objectives: To determine the minimum inhibitory concentration (MIC) of certain antibiotics against A. baumannii strains, and find the correlation between carbapenem resistance and the presence of carbapenemase encoding genes. Methods: 51 A. baumannii strains isolated from 6 hospitals across 3 regions in Vietnam. DNA extraction and purification, followed by next-generation sequencing. High-quality reads were de novo assembled using SPAdes 3.13.0 software, gene prediction and annotation were done using Prokka software. Results: 8 out of 9 antibiotics showed resistance above 70%, with 88% of the strains resistant to both carbapenem antibiotics, with MIC50 and MIC90 values of 32-64μg/ml and >64μg/ml respectively. The lowest resistance rate was seen with colistin (29%) and the MIC range for colistin was 1 - 8 μg/ml; MIC50 = 1 μg/ml, MIC90 = 4 μg/ml. Screening the antibiotic resistance genes of the 51 strains from 4 databases (ARG-ANNOT, NCBI, CARD, and ResFinder) resulted in 52 antibiotic resistance genes, including 22 genes encoding carbapenemases. The results showed an increasing MIC concentration in strains carrying multiple carbapenemase genes, with higher levels of resistance associated with higher MIC concentrations. Conclusions: There is a correlation between MIC and the presence of certain carbapenemase-encoding genes (blaOXA-23, blaOXA-66) in A. baumannii strains. Colistin has become the last line of defense in the treatment of multidrug-resistant A. baumannii infections.
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Copyright (c) 202 Journal of Science and Technology in Medicine and Pharmacy
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