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Abstract
Background: Soybean (Glycine max) is high-value grain crops recognized for their nutritional benefits and soil-improving capacity. However, its tolerance to drought and salinity is relatively low. Within the soybean genome, the AP2 gene family has been identified as playing a key role in activating the transcription of stress-responsive genes. Objectives: The aim was to evaluate the gene’s function and establish a foundation for developing transgenic soybean lines with enhanced tolerance to drought and salinity. Methods: In this study, a vector construct carrying the GmAP2 gene, which encodes a transcription factor protein of the AP2 family, was successfully introduced into Nicotiana tabacum cultivar K326 via Agrobacterium tumefaciens-mediated transformation. Transformed samples were regenerated in vitro, selected using antibiotic resistance, and cultured to produce complete plants. Results: Results showed that 62 leaf explants produced multiple shoots, forming 145 shoot clusters. Among these, 227 shoots were selected and elongated, with 112 successfully rooted. A total of 65 transgenic plants were acclimatized and transferred to the substrate; 29 plants survived and grew under greenhouse conditions. Conclusions: The pBI121-GmAP2 construct was successfully introduced into the K326 tobacco cultivar via Agrobacterium tumefaciens. Transformed explants were regenerated in vitro, selected using antibiotic screening, and subsequently developed into transgenic tobacco plants.
| Published | 2025-12-31 | |
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| Issue | Vol. 4 No. 5 (2025) | |
| Section | Original article | |
| DOI | 10.66517/jstmp.2025.5.3 | |
| Keywords | Biến nạp di truyền, GmAP2, Stress phi sinh học, Nhân tố phiên mã, Thuốc lá Genetic transformation, GmAP2, Abiotic stress, Transcription factor, Tobacco |
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